Cell-wall deficient L. monocytogenes L-forms feature abrogated pathogenicity

Cell-wall deficient L. monocytogenes L-forms feature abrogated pathogenicity

Stable L-forms are cell wall-deficient bacteria which are able to multiply and propagate indefinitely, despite the absence of a rigid peptidoglycan cell wall. We investigated whether L-forms of the intracellular pathogen L. monocytogenes possibly retain pathogenicity, and if they could trigger an innate immune response. While phagocytosis of L. monocytogenes L-forms by non-activated macrophages...

Cell-wall deficiencies in L-forms of Staphylococcus aureus.

Listeria monocytogenes L forms. I. Induction maintenance, and biological characteristics.

L forms were induced from 15 of 16 strains of Listeria monocytogenes on penicillin gradient plates incubated under aerobic conditions. The culture medium for maintenance of these L forms must contain an electrolyte in a concentration of 1% or sucrose in a concentration of 10%. The electrolytes NaCl, KCl, or MgSO(4) were used in both induction and maintenance media. Induction of L forms occurred...

The attachment efficiency of cell-walled and L-forms of Listeria monocytogenes to stainless steel

There is limited knowledge regarding the attachment of cell wall-deficient forms of Listeria monocytogenes to abiotic and food contact surfaces. A typical L-form phenotype of Listeria monocytogenes was induced, propagated and recovered in both broth and plate culture by exposure to sub-lethal concentrations of ampicillin. Attachment of both cell-walled and L-forms to stainless steel were compar...

Cell wall deficient forms of mycobacteria: a review

Cell wall deficient (CWD)-forms or L-forms of bacteria are characterized by a complete or partial loss of cell wall components and by the change of cellular shape. CWD-forms (spheroplasts) of bacteria are commonly prepared in vitro and are used for various practical applications. However, very little is known about the conditions required for the formation of CWD-forms of bacteria and mycobacte...